Signalling
Part:BBa_K4061070:Design
Designed by: Diya Agrawal Group: iGEM21_HKUST (2021-10-14)
pOmpF and pOmpC with reporters (TCS)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1918
Illegal AgeI site found at 2030 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since this part has two regulatory units, we had to reduce chances of recombination and DNA secondary structure. So, instead of using the commonly used double terminator, we used a short sequence of T0 terminator. We used strong RBS for both fluorescence proteins to express them in high intensity important for biosensor. The eGFP is overpowering the other reporter, so teams could perhaps swap out the eGFP to any other fluorophore that they prefer.
Source
The OmpF promoter was identified from BLAST search on E. coli K12 strain. -400bo from ORF of OmpF. The other parts are taken from biobricks